Our lab studies stem cell coordination, regulation, and malfunction from a quantitative and systems biology perspective. We use mouse hematopoietic stem cells as a model system and study individual stem cells collectively as a network in the context of development, regeneration, aging, and disease. Our investigations integrate research strategies from a broad range of disciplines including molecular biology, cell biology, systems biology, bioengineering, and bioinformatics. Our goal is to provide mechanistic insights into the regulation of the stem cell network, involving continuous cell fate transitions, single-cell lineage hierarchy, spatial interactions, and intercellular signaling. Our research can potentially lead to novel diagnostic tools and treatment regimens by pinpointing the contribution of individual cells and their network responses to diseases.